Figure 2
From: Consumption of oxygen: a mitochondrial-generated progression signal of advanced cancer
Reduction of the mitochondrial genome content in PCa and BCa constitutively activates Ras. (a) Activated (GTP-bound) Ras was pulled down and determined by western blotting. Negative controls (*GDP) and positive controls (**GTPγS): before pull-down, an aliquot of total protein lysate was pre-loaded with GDP or GTPγS, respectively. (b) Activated Ras in total-protein lysates (160 μg) of the indicated PCa cells lines. (c) DAB2IP in total-protein lysates (90 μg) of normal PrEC and the indicated PCa cell lines
