Figure 3

Reduction of the mitochondrial genome content in PCa overexpresses proto-oncogenic K-Ras 4A. (a) Ras isoforms in total protein lysates (30 μg) of the indicated cell lines. Exposure time was 10 s, excluding K-Ras 4A (0.5 s). (b) RT-PCR analysis of Ras (cDNA) transcripts in total-cDNA constructed from total-RNA lysates of the indicated cell lines. (c) K-Ras 4A in total-protein lysates (30 μg) of the indicated cell lines. (d) Phospho-ERK and phospho-Akt in total-protein lysates (30 μg) of LNρ0-8 transfected in the absence (control) or presence of K-RAS siRNA or non-specific siRNA. Protein levels of K-Ras 4A were evaluated in ^*(0.5 μg) total-protein lysates