Figure 4 | Cell Death & Disease

Figure 4

From: Protection of pancreatic INS-1 β-cells from glucose- and fructose-induced cell death by inhibiting mitochondrial permeability transition with cyclosporin A or metformin

Figure 4

Effects of 30 mM glucose or 2.5 mM fructose on INS-1 viability. INS-1 cells incubated in RPMI 1640 medium supplemented or not with 1 μM CsA for 1 h or 100 μM metformin for 24 h were then incubated in complete RPMI 1640 medium supplemented or not (control) with mannitol (osmotic control for glucose 30 mM), glucose (30 mM, final concentration) or 2.5 mM fructose for 72 h. Cell viability was assessed by double labeling as described in Materials and Methods. Histograms represent the results of three different experiments. Results are mean±S.E.; *P<0.05 versus control, #P<0.05 versus glucose 30 mM 24 h, §P<0,05 versus fructose 2.5 mM 24 h, unpaired Student's t-test

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