Table 1 Induction of apoptosis in SH-SY5Y cells by hPrP90-231

From: Human PrP90-231-induced cell death is associated with intracellular accumulation of insoluble and protease-resistant macroaggregates and lysosomal dysfunction

Days of treatment

1

2

3

4

α-hPrP90-231

102±0.3

112±0.21

133±0.2*

120±0.15*

β-hPrP90-231

127±0.19* O

190±0.13** OO

297±0.12** OO

250±0.3** OO

  1. hPrP90-231, in native structure (α-hPrP90-231) or in a β-sheet-rich conformation (β-hPrP90-231), was used at the concentration of 1 μM. Apoptosis was quantified by cytosolic oligonucleosome ELISA, after 1–4 days. Data were expressed as percentage of PBS-treated controls and represents the average of three independent experiments performed in quadruplicate
  2. *P<0.05, **P<0.01 versus cont. values. OP<0.05, OOP<0.01 versus samples treated with α-hPrP90-231
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