Table 2 Cathepsin D inhibition by pepstatin A inhibits SH-SY5Y cell death and induction of apoptosis induced by β-hPrP90-231

From: Human PrP90-231-induced cell death is associated with intracellular accumulation of insoluble and protease-resistant macroaggregates and lysosomal dysfunction

Treatment

Cell viability (% of control)

Apoptosis (% of control)

Control

100

100

β-hPrP90-231

68±0.5*

297±0.12**

β-hPrP90-231+pepstatin A

80±0.7* O

218±0.09** OO

Pepstatin A

99±0.2

108±0.06

  1. Cells were pretreated with pepstatin A (10 μM) at 1 h before β−hPrP90-231 (1 μM). Cell viability was measured by MTT reduction assay, and the extent of apoptosis was detected using cytosolic oligonucleosome ELISA
  2. Data were expressed as percentage of PBS-treated control cells and represents the average of three independent experiments performed in quadruplicate
  3. *P<0.05, **P<0.01 versus cont. values. OP<0.05, OOP<0.01 versus hPrP90-231-treated values
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