Figure 3 | Cell Death & Disease

Figure 3

From: Isthmin exerts pro-survival and death-promoting effect on endothelial cells through alphavbeta5 integrin depending on its physical state

Figure 3

ISM induces apoptosis through IMD. (A) ISM induced apoptosis through caspase-8-mediated extrinsic pathway, but not caspase-9-mediated intrinsic pathway. (B) Kinetics of ISM-induced apoptosis. Activated caspase-8 can first be detected 4 h after ISM treatment. (C) Knockdown of β5-integrin subunit by siRNA suppressed caspase-8 and caspase-3 activation, as well as cleavage of PARP. (D) ISM induces caspase-8 association with αvβ5 integrin and activation. (E) Soluble ISM induces αvβ5-integrin clustering. Co-localization of β5 integrin (red) with ISM (green) is indicated by yellow signals. DAPI (blue) stains the nucleus. Scale bar: 20 μm

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