Figure 5 | Cell Death & Disease

Figure 5

From: Isthmin exerts pro-survival and death-promoting effect on endothelial cells through alphavbeta5 integrin depending on its physical state

Figure 5

Immobilized ISM promotes EC adhesion through αvβ5 integrin. (A) ECs attach to ISM-coated surface in a dose-dependent and saturable manner. (B) Similar to VN, ISM supports EC adhesion and spreading, induces integrin clustering and actin stress fiber formation. Cell spreading in the absence or presence of EDTA are shown in phase contrast micrographs (panels A–D, scale bar: 40 μm). Actin stress fibers (red) and β5 integrin clustering (green) are shown in panels E–H (scale bar: 20 μm). (C) ISM mediates EC adhesion through αvβ5 integrin. (D) Knockdown of β3 and β5 integrin suppresses EC adhesion to VN. (E) Knockdown of β5 integrin suppresses EC adhesion to ISM

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