Figure 1
From: In vivo and in vitro assessment of pathways involved in contrast media-induced renal cells apoptosis
Immunohistochemistry of kidney tubular cells. (a) Cluster of tubular cells in an inflammatory background. The typical morphological features of tubular cells (regular elements with vacuolated and clear cytoplasm with eccentric nuclei) can be appreciated (Papanicolaou staining, × 400). (b) Urine cytological cell block preparation. This specimen type was used to perform specific tubular cell marker immunostaining. (Hematoxylin and eosin staining, × 400). (c) The presence of tubular cells was confirmed by a specific staining for the Gal-3 marker. (Haematoxylin counterstained, × 400). (d) The presence of distal tubular cells was confirmed by a specific staining for the CK7 marker. (Haematoxylin counterstained, × 400). (e) Active caspase 3 staining of samples from untreated patient. (f) Active caspase 3 staining from samples of patients upon 48 h of CM
