Figure 8

Production of reactive oxygen species (ROS) after contrast media treatment. MDCK cells were treated for 3 h with 50, 100 or 200 mgI/ml of iodixanol, iobitridol, iopamidol, and with 400 μM of H₂O₂ (positive control). (a) ROS formation on different CM. By ANCOVA test, ROS production was significantly increased in the presence of medium (100 mgI/ml) and high (200 mg/ml), but not at low (50 mgI/ml) dose of contrast media. ^*P>0.05 versus control; ^†P<0.030 versus control; ^‡P<0.001 versus control. (b) Effects of 2 h NAC (100 mM) pretreatment on ROS formation following contrast media exposure. ^†P<0.001 versus control; ^‡P<0.006 versus column without NAC