Figure 4 | Cell Death & Disease

Figure 4

From: The glycan-binding protein galectin-1 controls survival of epithelial cells along the crypt-villus axis of small intestine

Figure 4

Gal-1-mediated induction of enterocyte apoptosis in vivo. (a) Flow cytometry analysis of annexin V and PI staining in cells obtained from duodenal ligated loops. Results correspond to cells isolated from either wild-type or Lgals1−/− mice that had ligated loops filled with saline, 10 μM rGal-1, 20 μM rGal-1 or the same concentration of BSA as indicated. The histogram shows representative results out of four separate experiments: *P<0.05. (b) TUNEL assay of ligated loops corresponding to wild-type and Lgals1−/− mice filled with saline or rGal-1 20 μM. TUNEL-positive nuclei appear in cyan (arrows). Background shows DAPI staining. Results are representative of three independent experiments

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