Figure 7
Gal-1 triggers apoptotic death in human enterocytes. (a) Ethidium bromide and acridine orange staining of human enterocytes by fluorescence microscopy. Live enterocytes at initial time (left panel); isolated enterocytes exposed to 10 μM rGal-1 for 16 h where a majority of apoptotic enterocytes (brilliant green or orange nucleus) can be observed (middle panels) and necrotic enterocytes (right panel). (b) Flow cytometry analysis of annexin V and PI staining of enterocytes incubated with medium alone or 10 μM rGal-1. (c) Mean values of percentages of annexin V positive cells. *P<0.05. The results shown are representative of four independent experiments
