Table 1 Pairs of candidate interacting proteins were evaluated using the split YFP system for their ability to generate specific fluorescence upon induction of apoptosis in MCF7 cells
From: Utilizing mitochondrial events as biomarkers for imaging apoptosis
Protein pairs | YFP fluorescence | ||
|---|---|---|---|
C-YFP | N-YFP | Baseline | Apoptosis |
WD(APAF-1)–C-YFP | Cyt c–N-YFP | Low background | No change |
XIAP–C-YFP | Smac–N-YFP | Low background | No change |
C-YFP–Bax | MitoPLD–N-YFP | Low background | No change |
C-YFP–Bax | Cyt c–N-YFP | Low background | High increase in mitochondria |
C-YFP–Bax | Smac–N-YFP | Low background | High increase in mitochondria |
C-YFP–Bax | N-YFP–Bax | Low background | High increase in mitochondria |
Bid–C-YFP | N-YFP–Bax | High background in cytosol | Translocation to mitochondria |
