Figure 3 | Cell Death & Disease

Figure 3

From: Transcription factors that mediate epithelial–mesenchymal transition lead to multidrug resistance by upregulating ABC transporters

Figure 3

Dox treatment of invasive cells leads to the upregulation of EMT markers and invasiveness. (a) Graphs represent relative mRNA expression of EMT markers after 1-week Dox treatment in MCF10A, MCF7, and MDAMB231 cells, n=3. Error bars denote ±S.E.M. *P<0.05. (b) Immunoblot analysis of whole-cell lysates of MCF10A, MCF7, and MDAMB231 cells for the expression of EMT markers in the absence or presence of Dox. (c) Photomicrographs represent cell migration of untreated (UT) or doxorubicin-treated (Dox) MDAMB231 cells in wound healing assay at 0 and 24 h. Scale bar=500 μm. (d) Graph represents distance migrated by cells in the experiment (c) calculated as a difference of distance between the two edges of the wounds at 0 and 24 h measured in μm using the ProgRes capture software, n=8. Error bars denote ±S.E.M. **P<0.01. (e) Graph represents relative mRNA expression of MMP markers after 1 week of Dox treatment in MDAMB231 cells, n=3. Error bars denote ±S.E.M. *P<0.05; **P<0.01. (f) Graph represents invasion index calculated as ratio of percentage cell invasion of Dox-treated cells and -untreated control cells through Matrigel in Boyden chamber assay; n=2 for MCF10A and MCF7, and n=3 for MDAMB231. Error bars denote ±S.E.M. *P<0.05

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