Figure 7
Effect of a combination of nutlin-3 and ABT-737 on the induction of apoptosis in EBV (+) Burkitt's lymphoma (BL) and lymphoblastoid cell lines (LCL). (a) EBV (+) cell lines were or were not subjected to prior treatment with ABT-737 (BL2/B95, Seraphina, LY47: 10 μM; 8866, Priess, Remb1: 0.25 μM) for 1 h and were then left untreated or treated with nutlin-3 for 24 h. The cells were labeled with annexin V-FITC and PI and analyzed with a FACSCalibur flow cytometer, to determine the percentage of cells that were apoptotic. The values presented (means±s.d.) are from three independent experiments. (b) Cell lines were treated as in (a) and apoptosis levels were determined by assessing polyadenosine diphosphate-ribose polymerase (PARP) cleavage on western blots. Results are representative of at least three independent experiments
