Figure 5
From: Apical membrane rupture and backward bile flooding in acetaminophen-induced hepatocyte necrosis
Three kinds of APAP-injured cells based on the staining of the mitochondrial dye R6G. In addition to R6G, CFDA and Sytox were also injected. Cells indicated by arrows in A, B, and C were serially observed. Kinetics of CF and R6G in these cells were shown in D. (A) A cell not labeled by R6G but stained by Sytox developed apical membrane rupture later. (B) A cell originally well labeled by R6G developed simultaneously bile flooding into cell and loss of R6G staining. (C) A Sytox-stained cell recovered its R6G labeling. (D) Kinetics of CF and R6G for these three cells in comparison with normal hepatocytes. Plot a, b, and c are for the indicated cells in A, B, and C, respectively. Note resurgence of CF fluorescence (arrows) in a and b, simultaneous drop of R6G fluorescence in b and re-elevation of R6G fluorescence (arrow) in c. Scale bars indicate 20 μm
