Figure 1

Properties of transfected HaCaT CYLD cells. (a) Western blot showing CYLD overexpression in one of the H-CYLD^WT pools (H-Co, H-Control cells). Tubulin was used as loading control. (b) H-control and H-CYLD^WT cells differentiation cultures. Red circles: stratification domes (c) (top) keratin K10 RNA levels in H-control and H-CYLD^WT cells detected by northern blotting at the stated times (days); 7S, loading control. (bottom) Graphical representation of the northern blotting signals relative to those of loading controls (arbitrary units). The difference in K10 content between cells of both genotypes is statistically significant at 6, 10 and 13 days of differentiation. (d) Western blot of proteins from H-control, H-CYLD^WT and H-CYLD^C/S cells in the basal state or after UV stimulation showing an increase in JNK activation in the H-CYLD^C/S cells and a reduction in the H-CYLD^WT. Analysis of Bax expression. Actin was used as loading control. Western blots were performed 3–4 times