Figure 4

Characterization of A-CYLD^WT and A-CYLD^C/S cells. (a) Western blot analysis of CYLD and Bax expression in total protein extracts from A431 cells of the three genotypes. (b) Monolayer culture of A-control, A-CYLD^WT and A-CYLD^C/S cells. Red circles: foci of spontaneous differentiation. Note the scattered phenotype of A-CYLD^C/S cells and its loose growth in culture. (c) Immunofluorescence showing expression of K10 in A-CYLD^WT cells. (d) Western blot of protein extracts from A-control, A-CYLD^WT and A-CYLD^C/S cells in unstimulated or after UV stimulation. Note the increase in JNK activation in A-CYLD^C/S cells and the reduction in the A-CYLD^WT cells