Table 2 Neurological benefit at 48 h: effect of TRP601 (i.v., 1 mg/kg, 1 h post-ischemia) on general behavioral profiles

From: Targeting neonatal ischemic brain injury with a pentapeptide-based irreversible caspase inhibitor

 

Naïve ( n =20)

Isch.+vehicle ( n =20)

Isch.+TRP601 ( n =20)

P-values ( χ2) (n =20)

Spontaneous activity

1.8±0.4

0.3±0.6

1.1±0.6

0.026 × 10−6

Walking (circle versus straight)

0.3±0.4

1.6±0.6

0.8±0.6

1.092 × 10−6

Reaction to pain

1.7±0.6

0.7±0.6

1.2±0.6

95.33 × 10−6

Paw withdrawal

1.6±0.6

0.3±0.6

1.2±0.6

0.261 × 10−6

Mean lesion volume (mm3)

0

24.3±2.5

9.2±2.3

NA

  1. Sensorimotor neurological deficits were assessed in a blinded manner in 7-day-old rat pups. Animals were subjected to ischemia–reperfusion (as in Figures 2d–f) and treated with 1 mg/kg TRP601 (i.v., 1 h post-ischemia). At 48 h post-ischemia, pups were tested for the following neurological signs and reflexes: (i) spontaneous activity (spontaneous postural signs such as right forelimb flexion and thorax twisting and exploration of the cage); (ii) walking (after ischemia pups walk in circle rather than straight); (iii) reaction to pain (the pup escapes after the tail is pinched); (iv) paw withdrawal (pups withdraw paw from adhesive pad). Grading scale of neurological examination for each test item was: 2 for normal; 1 for intermediate; and 0 for abnormal. A neurological score (mean±S.E.M.) was attributed for each test in each experimental condition (d.o.f. stands for degree of freedom in the χ2 statistical test). NA, not applicable
Back to article page