Table 3 Physiological variables in TRP601-treated rat pups

From: Targeting neonatal ischemic brain injury with a pentapeptide-based irreversible caspase inhibitor

Parameters

Vehicle

TRP601

Blood hemodynamics and biochemistry

 Blood flow (n=5, ml/min)

1 h

4.2±0.9

3.6±0.4

 Resistance indexa (n=8)

1 h (cerebral artery)

0.68±0.01

0.64±0.03

 Bleeding time (n=10)

1 h

319±34

395±103

 Arterial pH (n=8)

1h

7.42±0.03

7.42±0.02

 PaO2 (n=8, mm Hg)

1 h

70.84±6.38

69.82±2.24

 PaCO2 (n=8, mm Hg)

1 h

37.96±2.64

42.06±2.58

 HCO3− (n=8, mM)

1 h

24.16±0.61

26.30±0.76

Cardiac physiology

 Heart rate (n=8, beat/min)

1 h

345±13

344±12

 LVEDd (n=8, mm)

1 h

0.293±0.015

0.310±0.015

 LVO (n=8, ml/min)

1 h

0.135±0.009

0.157±0.015

 Fractional shortening (n=8, %)

1h

54.39±0.96

49.97±2.11

 VTIm (n=8)

1 h

4.05±0.11

3.92±0.29

 Core temperature (n=10, °C)

1 h

36.60±0.28

36.40±0.67

3 h

36.39±0.54

36.40±0.67

48 h

36.63±0.23

36.80±0.23

 Body weight (n=10, g)

Co.

19.49±1.43

19.18±0.95

1 h

19.35±1.72

20.40±1.67

48 h

20.96±1.25

20.90±0.67

14 days

69.56±1.60

62.00±2.50

  1. Blood hemodynamics, biochemistry, cardiac physiology, temperature, and body weight were evaluated before and 1 h after TRP601 (1 mg/kg) or vehicle injection. Anterior cerebral arteries were located by transcranial color-coded duplex sonography to measure peak systolic (Vs) and maximum end-diastolic (Vd) velocities. Resistive index (RI) was calculated as follows: RI=((Vs−Vd)/Vs), and corresponds to an index of cerebral perfusion. For cardiac physiology, the left ventricle (LV) was imaged in short axis view, and diastolic diameter (LVEDd) was defined as the largest LV area. The fractional shortening was calculated with the following formula: FS (%)=((LVEDd−LVEDs)/LVEDd) × 100. LVED corresponds to left ventricular end-systolic diameter. A pulsed-wave Doppler spectrum of aortic outflow was recorded from suprasternal view to evaluate left ventricular output (LVO). Velocity–time integral (VTI) of aortic flow, aortic root diameter (CSA), and heart rate (HR) were measured to calculate LVO as follows: LVO=CSA × VTI × HR. A pulsed-wave Doppler spectrum of mitral inflow (VTIm) was recorded from the apical four-chamber view as an index of diastolic function. Statistical analysis was performed by a mean comparison using a Scheffe test.
  2. aTranscranial Doppler sonography
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