Figure 2 | Cell Death & Disease

Figure 2

From: Spontaneous formation of IpaB ion channels in host cell membranes reveals how Shigella induces pyroptosis in macrophages

Figure 2

IpaB kills phagocytes but not epithelial cells. (a and b) Cytotoxicity of IpaB was quantified by measuring LDH release from macrophages after 2 h of treatment. Cells treated with indicated IpaB concentrations (a) or 0.8 μM IpaB, IpaB/IpgC or IpgC (b). (c) Kinetics of IpaB cytotoxicity in BMMs and HeLa cells detected by recording fluorescence of DNA-incorporated Sytox Green (Invitrogen, Darmstadt, Germany) dye at 518 nm for 2 h. (d) Cytotoxicity of IpaB expressed as % of totally lysed cells with Triton X-100. Cell death was detected as described in (c) and the values present cytotoxicity after 2 h of treatment with IpaB. Values are the average of triplicates±S.D. Results are representative of at least two independent experiments. See also Supplementary Figure 2

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