Figure 3 | Cell Death & Disease

Figure 3

From: Therapeutic metformin/AMPK activation blocked lymphoma cell growth via inhibition of mTOR pathway and induction of autophagy

Figure 3

Metformin-induced mTOR inhibition in an AMPK-dependent manner. (a) Metformin decreased the expression of phosphorylated mTOR, as well as its effectors p70S6K and 4EBP1. (b and c) Specific AMPK inhibitor compound C (b) and AMPK siRNA (c) abrogated metformin-induced lymphoma cell growth inhibition and cell cycle arrest. Comparing with the negative control (Con siRNA), Jurkat cells transfected with AMPK-α siRNA (AMPK siRNA) failed to induce phosphorylated ACC and inhibit phosphorylated mTOR. *P<0.001 comparing with the metformin group. +P<0.001 comparing with the Con siRNA group. (d) The expression of phosphorylated AKT remained unchanged during metformin treatment in Jurkat cells. (e) Cell sensitivity to metformin was not affected in Jurkat cells transfected with AKT

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