Figure 2 | Cell Death & Disease

Figure 2

From: NHERF1/EBP50 controls lactation by establishing basal membrane polarity complexes with prolactin receptor

Figure 2

Impaired mammary gland development correlates with lack of NHERF1 upregulation during pregnancy. (a) Whole-mount analysis by carmine alum staining of mammary glands from age-matched NHERF1(+/+), (+/−) and (−/−) virgin females and dams at ≤24 h postpartum. Boxed numbers indicate the success rates for nursing six or more pups (from Figure 1b). (b) Hematoxylin and eosin staining of contralateral mammary glands from lactation success and lactation failure mothers at × 100 and × 200 magnification. Myoepithelial cells are shown with arrowheads. (c) Western blot of mammary gland lysates shows NHERF1 upregulation in lactation success glands. Proteins were loaded at 100 μg per lane. (d) NHERF1 mRNA analysis by RT-PCR (panels) or quantitative real-time PCR (graph). n=3. (e) Western blot with NHERF2 antibody shows compensatory NHERF2 upregulation in NHERF1(+/−) and (−/−) success glands. NS, nonspecific band

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