Figure 1 | Cell Death & Disease

Figure 1

From: Remnant living cells that escape cell loss in late-stage tumors exhibit cancer stem cell-like characteristics

Figure 1

In vitro functional analysis of reporter genes transduced into 4T1 cells using the piggyBac transposon system. (a) Tracking of mRFP expression in sorted 4T1-PB-2R/PBase and 4T1-PB-2 R cells in a time-course manner. (b) Western blot analysis of HSV1-tk expression in mRFP-sorted cells cultured for 60 days. (c) Cell-uptake assay to detect HSV1-tk activity in cells treated with 3H-FIAU. The data represent the means of three independent experiments±S.D. *P<0.05. (d) FISH assay for visualizing the integration of the mRFP reporter gene in the chromosomes of 4T1-PB-2R/PBase cells (the mean copy number was approximately 14 by counting six cells). (e) Genomic PCR for amplifying the mRFP DNA sequence from cellular chromosomes. P.C., a positive control by amplifying the mRFP gene from the original PB-2R-puro plasmid. The PCR product was 0.67 kb

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