Figure 5

Expression of CSC-related markers in ex vivo isolated remnant living cells. (a) Oct4 mRNA and (b) Sox2 mRNA expression was compared between cultured and ex vivo isolated remnant living 4T1-PB-2R/PBase cells using qPCR. (c) Luciferase reporter-gene assay for investigating the Oct4 transcriptional activity in parental cells and isolated remnant living cells. The data represent the means of four independent experiments±S.D. *P<0.05. (d) Western blot analysis to compare the expression of Oct4, Sox2, and CD133 proteins between cultured cells and four ex vivo isolated remnant living cells