Figure 2

HMQ18–22 inhibits angiogenesis in the CAM and colon tissues. (a) The representative images of CAM blood vessels ( × 40 magnification): in the control group, the blood vessels grew normally; CAM blood vessels in the HMQ18–22 (2.5, 5, 10 μg/egg)-treated group did not increase as quickly as that of the untreated group. (b) Quantification of (a): compared with the negative control group, HMQ18–22 showed the inhibitory effect on the normal CAM angiogenesis in a dose-dependent manner. *P<0.05 versus the untreated control group. (c1–c8) HMQ18–22 inhibited microvessel outgrowth of cultured colon tissues ( × 100 magnification). (c1–c5) The untreated control group (c1) day 5; (c2) day 7; (c3) day 9; (c4) day 11; (c5) The high magnification of the inset on the 11th day; (c6–c8) in the HMQ18–22-treated group, (c6) 4.0 μmol/l HMQ18–22 on day 5; (c7) 4.0 μmol/l HMQ18–22 on day 11; (c8) The high magnification of the inset treated with 4.0 μmol/l HMQ18–22 on day 11. Scale bar, 100 μm. (d) Quantification of microvessels in the control group with the increased days. (e) Quantification of microvessels in the HMQ18–22-treated group. Microvascular number indicated the microvessel number grown from the rat colon tissues. Data were expressed as mean values±S.D. (n=5). *P<0.05, **P<0.01 versus the untreated control group