Figure 1 | Cell Death & Disease

Figure 1

From: Raf kinases mediate the phosphorylation of eukaryotic translation elongation factor 1A and regulate its stability in eukaryotic cells

Figure 1

B-Raf kinase assay of eEF1A1-His and eEF1A2-His. (a) Autoradiography of the assay on eEF1A1-His in the presence of B-Raf wt (10 ng/μl) and B-Raf V600E (10 ng/μl), respectively. Lanes: 1, B-Raf wt (diluted 1 : 5) + eEF1A1-His; 2, B-Raf wt + eEF1A1-His; 3, B-Raf V600E (diluted 1 : 5) + eEF1A1-His; 4, B-Raf V600E + eEF1A1-His; 5, B-Raf wt; 6, eEF1A1-His and 7, B-Raf wt + Mek + ERK. (b and c) Immunoblotting against antibody (Ab) anti-eEF1A and anti-B-Raf, respectively. (d) Autoradiography of the assay on eEF1A2-His in the presence of B-Raf wt (10 ng/μl). Lanes: 1, B-Raf wt (diluted 1 : 5) + eEF1A2-His; 2, B-Raf wt + eEF1A2-His; 3, B-Raf wt; 4 and B-Raf wt + Mek + ERK. (e and f) Immunoblotting against Ab anti-eEF1A and anti-B-Raf, respectively. (g) Autoradiography of the assay on eEF1A1-His in the presence of B-Raf wt (20 ng/μl). Lanes: 1, B-Raf wt (diluted 1 : 5) + eEF1A1-His; 2, B-Raf wt + eEF1A1-His; 3, eEF1A1-His; 4, B-Raf wt and 5, B-Raf wt + Mek + ERK. (h and i) Immunoblotting against Ab anti-eEF1A and anti-B-Raf, respectively. (k) Autoradiography of the assay on eEF1A2-His in the presence of B-Raf wt (20 ng/μl). Lanes: 1, B-Raf wt (diluted 1 : 5) + eEF1A2-His; 2, B-Raf wt + eEF1A2-His; 3, B-Raf wt + Mek + ERK; 4, B-Raf wt and 5, eEF1A2-His

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