Figure 3 | Cell Death & Disease

Figure 3

From: Raf kinases mediate the phosphorylation of eukaryotic translation elongation factor 1A and regulate its stability in eukaryotic cells

Figure 3

Expression levels of GST-eEF1A1, eEF1A1-HIS and eEF1A2-HIS and corresponding mutants in COS 7 cells. (a, g and d) Western blots of cell lysates with antibody anti-His and anti-GST, respectively. (a) Lanes: 1, eEF1A1-HIS wt; 2, eEF1A1-HIS S21A; 3, eEF1A1-HIS S21D; 4, eEF1A1-HIS T88A; 5, eEF1A1-HIS T88D. (d) Lanes: 1, GST-eEF1A1 wt; 2, GST-eEF1A1 S21A; 3, GST-eEF1A1 S21D; 4, GST-eEF1A1 T88A; 5, GST-eEF1A1 T88D. (g) Lanes: 1. eEF1A2-HIS wt; 2, eEF1A2-HIS S21A and 3, eEF1A2-HIS S21D. (b, e and h) Relative band intensity evaluation. (c, i and f) Western blots of cell lysates with antibody anti-β-actin and anti-GAPDH, respectively. Each measurement and western blot was carried out in triplicate. Error bars indicate the maximum deviation from the mean value of two independent experiments

Back to article page