Figure 5 | Cell Death & Disease

Figure 5

From: Raf kinases mediate the phosphorylation of eukaryotic translation elongation factor 1A and regulate its stability in eukaryotic cells

Figure 5

Effects of transfection with eEF1A-HIS isoforms and their S21 mutants on apoptosis in H1355 cells. FACS analysis after double labelling with annexin V and propidium iodide of H1355 cells transfected with eEF1A-HIS constructs for 72 h as described in Materials and Methods and Supplementary Information. White bars: percentage of annexin V-FITC and PI positive (late apoptosis). Black bars: percentage of annexin V-FITC positive (early apoptosis). Samples: 1, control cells; 2, cells treated with lipophectamine; 3, cells transfected with void vector; 4, eEF1A1-HIS wt, 5, eEF1A1-HIS S21A; 6, eEF1A1-HIS S21D; 7, eEF1A2-HIS wt, 8, eEF1A2-HIS S21A and 9, eEF1A2-HIS S21D. Error bars indicate the maximum deviation from the mean value of two independent experiments

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