Figure 2 | Cell Death & Disease

Figure 2

From: Apoptosis and necrosis: two different outcomes of cigarette smoke condensate-induced endothelial cell death

Figure 2

CSC-induced DNA damage and mitochondrial depolarisation without caspase-3 activation. (a) Shows data from a DNA damage analysis by the comet assay. Percent comet-positive cells compared with the control are shown. (b) Shows western blot analysis of P53 in HUVECs treated with 50 or 100 μg/ml CSC after 24, 48 and 72 h, respectively. Representative blots are shown. Data in (c) shows JC-1-based FACS analysis of intracellular mitochondrial membrane potential after CSC treatment for 2, 6, 12 and 24 h. Mean values±S.D. of a representative experiment performed in triplicates (JC-1 staining) or quadruplicates (Comet assay) are shown. (d) Shows western blot analysis of caspase-3 in CSC-treated endothelial cells. The cells were incubated with 50 or 100 μg/ml CSC for 24, 48 and 72 h. The right western blot in (d) shows analysis of endothelial cells treated with ursolic acid (6.25 and 12.5 μM for 13 h), which serves as a positive control. Representative blots are shown. Mean values±S.D. are shown. Asterisks indicate significant differences (*P<0.05; **P<0.01; ***P<0.001) compared with the controls

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