Figure 4

Stimulation of pW224 transgene by gonadotropin. Three-week old Tg control (Tg-eCG) and eCG-stimulated (Tg+eCG; 5 IU per mouse) Tg ovaries were stained for β-galactosidase expression as wholemounts (a and b) and then serial sectioned (c and d). Ectopic expression of Umodl1-BAC is observed in the cGCs of the stimulated ovarian follicles (d). Scale bars in sections represent 50 μm. Regulation of Umodl1 by gonadotropin was tested in vivo by western blot assay on WT and Tg oocytes (e) and cumulus cells (g), and quantified by NIH ImageJ (f and h, respectively).Ovarian cells from untreated mice were included as controls. β–tubulin was used as sample loading control