Figure 2 | Cell Death & Disease

Figure 2

From: Cordyceps militaris and mycelial fermentation induced apoptosis and autophagy of human glioblastoma cells

Figure 2

Induction of hypodiploidic DNA contents and Annexin V positivity in human glioblastoma cells. Cultured human glioblastoma cells, GBM8401 (a) and U-87MG (b), were treated with either CM or mycelial fermentation (MF) for 24 h at the dose indicated, followed by propidium iodide (PI) staining and subsequent flow cytometric detection. The representative sub-G1 histogram profiles from three independent experiments are shown. (c) GBM8401 cells were treated with MF for indicated duration and subjected to Annexin V-FITC and PI detection by using flow cytometry. The percentage of sub-G1 and Annexin V-positive fractions in total cell populations is measured as mean±S.D. NC, negative control

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