Figure 4

Modulatory effects of mycelial fermentation (MF) of CM on regulator expression of cell cycle, apoptosis, and autophagy in human glioblastoma cells. GBM8401 and U-87MG cells were treated with MF at the indicated doses for 24 h, and the lysates were subjected to western blotting detection. (a) MF induced a significant upregulation of p53 and its downstream p21 expression in GBM8401, but not U-87MG cells. (b) MF treatment diminished the expression of antiapoptotic Bcl-2 protein, and conversely increased the abundance of proapoptotic Bax protein in both glioblastoma cells. (c) MF reduced mTOR expression but increased Atg5 and LC3 II levels in GBM8401 cells. Although MF upregulated mTOR content, MF did not affect the levels of autophagic regulators in U-87MG cells. The representative result of three independent experiments is shown