Figure 3
MiR-203 inhibits proliferation and induces G0/G1 arrest in HEKn by RAN and p63 downregulation. (a) At 48 h after transfection with scramble control or pre-miR-203 sequences, HEKn cells were subjected to a 4 h BrdU-pulse, and then collected, PI-stained and analyzed by flow cytometry. BrdU-positive cells are indicated as the S phase (fluorescent populations are discriminated for PI-stained DNA content, respectively, of 2n or 4n fixed to values of 200 and 400 in the plots). (b) Histogram shows the relative percentage of BrdU-incorporating cells (BrdU-positive cells). Results are shown as the mean±S.D. from three independent experiments
