Figure 4
Silencing by siRNA of miR-203 targets reduces the migratory potential of HEKn. (a) In vitro wound assay of HEKn cells, 48 h after transfection with scramble control (Ctr) or pre-miR-203 sequences. The rate of cell migration across the scratched area was monitored for 24 h and phase-contrast images are shown. (b) Histogram represents the relative percentage of distance covered by migration 24 h after scratch (% closure). Values reported were obtained as the average±S.D. of three different measures from three independent assays. (c) Western blot analysis of miR-203 target protein levels in HEKn cells at 48 h post-transfection with scramble control or target-specific siRNA. β-Actin was used as a loading control
