Figure 1 | Cell Death & Disease

Figure 1

From: miR-29b sensitizes multiple myeloma cells to bortezomib-induced apoptosis through the activation of a feedback loop with the transcription factor Sp1

Figure 1

miR-29b expression in MM primary samples and cell lines. (a) miR-29b expression levels obtained by Agilent-miRNA profiling of 723 human and 76 human/viral miRNAs. Histogram bars represent miR-29b expression values in 38 MM and 2 PCL patients, normalized by aroma.light-package for Bioconductor. (b) Quantitative RT–PCR analysis of miR-29b using total RNA from 10 MM cell lines. Raw Ct values were normalized to RNU44 housekeeping snoRNA and expressed as ΔΔCt values referenced to miR-29b levels in PBMC. Values represent mean of three different experiments±S.E. (c) Quantitative RT–PCR of miR-29b in NCI-H929 cells co-cultured with MM patient-derived BMSCs and then immunopurified by immunomagnetic sorting with anti-CD138 beads. Raw Ct values were normalized to RNU44 housekeeping snoRNA and expressed as ΔΔCt values calculated using the comparative cross threshold method. miR-29b levels in NCI-H929 harvested after 6 h of co-culture were set as internal reference. Data are the average of two independent experiments performed in triplicate. P-values were obtained using two-tailed t test. *P<0.01

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