Figure 3 | Cell Death & Disease

Figure 3

From: miR-29b sensitizes multiple myeloma cells to bortezomib-induced apoptosis through the activation of a feedback loop with the transcription factor Sp1

Figure 3

miR-29b expression in MM cells downregulates CDK6 and MCL-1 levels. Quantitative RT–PCR of MCL-1 and CDK6 24 h after transfection with synthetic miR-29b or scrambled oligonucleotides (NC) in SKMM1 (a) and NCI-H929 cells (c). The results are shown as average mRNA expression after normalization with GAPDH and ΔΔCt calculations. Data represent the average of three independent experiments±S.D. Immunoblot of MCL-1, CDK6, and Ser780-Rb phosphorylated 24 h after transfection with synthetic miR-29b or scrambled oligonucleotides (NC) in SKMM1 (b) and NCI-H929 cells (d). The protein loading control was performed using GAPDH. (e) Quantitative RT–PCR of miR-29b in SKMM1 cells after transduction with a lentivirus carrying the empty vector or antagomiR-29b (anti-miR-29b). Raw Ct values were normalized to RNU44 housekeeping snoRNA and expressed as ΔΔCt values. (f) Quantitative RT–PCR of MCL-1 and CDK6 in SKMM1 cells transduced with the empty vector (indicated as vector) or antagomiR-29b (anti-29b). The results are shown as average mRNA expression after normalization with GAPDH and ΔΔCt calculations. *P<0.001; •P<0.05

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