Figure 4 | Cell Death & Disease

Figure 4

From: miR-29b sensitizes multiple myeloma cells to bortezomib-induced apoptosis through the activation of a feedback loop with the transcription factor Sp1

Figure 4

miR-29b-enforced expression has anti-tumor activity in a MM mouse model. (a) In vivo tumor formation of NCI-H929 xenografts stably expressing miR-29b after lentivirus transduction with the empty vector (pMIF) or miR-29b (pMIF-miR-29b). Transduced cells were subcutaneously inoculated in the interscapular area of mice and tumor volumes were measured by an electronic caliper in two dimensions every 2–3 days starting the appearance of a palpable tumor mass. The picture inserted in the graph shows the in vivo detection of the tumor mass in a representative pMIF or pMIF-miR-29b NCI-H929-xenografted mouse using IVIS LUMINA II Imaging System (Caliper Life sciences). *P<0.0001. (b) Survival curves (Kaplan–Meier) show prolongation of survival of miR-29b (PMIF-miR-29b) xenografted animals compared with the empty vector (PMIF) control (log-rank test, P<0.05). (c) Quantitative RT–PCR analysis of miR-29b using total RNA from retrieved NCI-H929 xenografts. Raw Ct values were normalized to RNU44 housekeeping snoRNA and expressed as ΔΔCt values. Values represent mean of three different experiments±S.D. (d) Histology (H&E, original magnification, × 40), Caspase 3 and Ki-67 immunohistochemical staining (original magnification, × 20) of NCI-H929 tumor xenografts

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