Figure 3
From: PRAP1 is a novel executor of p53-dependent mechanisms in cell survival after DNA damage
Knockdown of PRAP1 sensitizes cells to DNA-damaging agents. HCT116 cells were transfected with PRAP1-specific siRNAs (PRAP1 siRNA1 and 2) or PRAP1 siRNA specific control siRNAs (control siRNA1 and 2) for 48 h and followed by the addition of 5-FU for another 24 h. PRAP1 knockdown significantly reduced 5-FU-induced PRAP1 mRNA and protein expression. (a and b) PRAP1 knockdown by siRNA reduces PRAP1 mRNA and protin expression induced by 5-FU treatment. (c) Knockdown of PRAP1 expression enhances 5-FU-induced DNA damage. PRAP1 knockdown increased the tail movement of 5-FU-treated cells (left panel), as well as the number of micronuclei (MN) formation (right panel), indicating enhanced DNA damage in these cells. (d) Knockdown of PRAP1 expression reduces colony numbers after 5-FU treatment. HCT116 cells were treated as described above with 3 μM or 6 μM 5-FU before re-culturing for colony formation assay. Colonies were stained with crystal violet for visualization and analysis using ImageJ. PRAP1 knockdown reduced the number of colonies formed by cells treated with both doses of 5-FU. (*P<0.05). GAPDH, glyceraldehyde 3-phosphate dehydrogenase
