Figure 5

Functional collaboration between PAK4 and MMP-2 in the regulation of αvβ3/EGFR-mediated migration and invasion in glioma. (a) 4910 and 5310 cells were treated with mock, pSV and PAK4si as described in Materials and Methods. At 24 h post-transfection cells were further treated with EGF (2 μg/ml) and GW2974 (10 μM) and incubated for another 24 h. Western blotting was performed using whole-cell lysates and GAPDH served as loading control. (b) Combination treatments were performed with VN (5 μg/ml) and αvβ3 integrin-blocking antibody (5 μg/ml) as described above and representative blots form three independent experiments were shown. (c) Percentage of cell invasion obtained from three independent experiments were plotted in the bar diagram (*P<0.05 and **P<0.01). (d) The relative migration distance values in wound-healing migration assays were plotted as mean±S.E. values obtained from three individual repetitions (*P<0.05 and **P<0.01)