Figure 6
Codepletion of PAK4 and MMP-2 significantly decreased cell invasion and migration and resulted in robust anoikis in glioma xenograft cells. (a) Western blotting was performed using whole-cell lysates, and GAPDH was used to monitor equal loading. (b) Percentage of cell viability was determined using CytoTox-Glo Cytotoxicity Assay kit and represented as mean±S.E. values obtained from three experimental replicates (*P<0.05, **P<0.01 and ***P<0.001). (c) FACS analysis shows the percent of apoptotic cells (sub-G1 %) was determined from three individual experiments. (d) The percentage of cell invasion was determined from three repetitions and was plotted in the bar diagram as mean±S.E. (*P<0.05 and **P<0.01). (e) The mean±S.E. of relative migration distance values obtained from three experimental replicates were plotted in the bar diagram and significant difference was denoted by * and ** at P<0.05 and P<0.01, respectively
