Figure 7

PAK4si suppressed orthotopic tumor growth. (a) In vivo intracranial tumor experiments were performed with pSV and PAK4si plasmids and representative pictures of tumor growth were presented (n=10). (b) The relative brain tumor size in pSV and PAK4si treatments was plotted as mean±S.E. (n=10, *P<0.01). (c) Immunohistochemical analysis was carried out in pSV- and PAK4si-treated tumors. Nuclei were counterstained with hematoxylin. (d) The subcutaneous tumor lysates were subjected to western blotting and representative blots from three independent experiments was presented. (e) Immunofluorescence staining depicts the expression and co-localization levels of PAK4 (red) and MMP-2 (green) in pSV- and PAK4si-treated 4910 and 5310 tumor sections. Nuclei were counterstained with DAPI. Insets: 4910 tumor sections stained with non-specific rabbit IgG as negative control. (f) TUNEL assay in pSV- and PAK4si-treated tumor sections (n=10). Nuclei were counterstained with DAPI