Figure 4
Paracetamol induced Bim expression is JNK-dependent. skHep-1 or EA.hy926 cells were pretreated (60 min) with increasing concentrations of the JNK inhibitor V (1, 3 and 10 μM) and stimulated with 10 mM paracetamol for 6 h. bim-mRNA expression was measured by quantitative RT-PCR. GAPDH was used to normalize bim-expression levels (a). skHep-1 and EA.hy926 cells were treated as described above and Bim protein expression was analyzed by western blot. Tubulin was used to normalize protein loadings (b). After pretreatment with 3 μM JNK inhibitor V, cells were stimulated with 10 mM paracetamol and 30 ng/ml, respectively 3 ng/ml TRAIL, or the combination thereof for 6 h and DEVD cleavage was measured (c). Mean values±S.D. triplicates are shown for quantitative RT-PCR and DEVDase assay. A typical experiment out of three is shown for western blot analysis. **P<0.01 versus paracetamol treatment, ***P<0.001 versus paracetamol treatment
