Figure 3

PIO effects on body weight. Graphs show time course of body weight changes monitored during the 9-month treatment in treated and untreated (a) WT, (b) PS1-KI, and (c) 3xTg-AD mice. Upper panels show weight changes in female mice; lower panels depict changes in males. All untreated genotypes showed a physiological time-dependent gain in body weight. PS1-KI mice and male 3xTg-AD mice gained significantly more weight compared with WT mice (CTRL-diet female PS1-KI versus CTRL-diet female WT, P=0.002; CTRL-diet male PS1-KI versus CTRL-diet male WT, P=0.01; CTRL-diet male 3xTg-AD versus CTRL-diet male WT, P=0.001). PIO treatment induced a significant time-dependent increase in body weight in all the treated genotypes. Enhanced PS1-KI gain in weight was observed when comparing these animals with PIO-diet WT mice (PIO-diet female PS1-KI versus PIO-diet female WT, P=0.004; PIO-diet male PS1-KI versus PIO-diet male WT, P=0.001) or when comparing with PIO-diet 3xTg-AD mice (PIO-diet female PS1-KI versus PIO-diet female 3xTg-AD, P=0.008, and PIO-diet male PS1-KI versus PIO-diet male 3xTg-AD, P=0.010). PIO effects on PS1-KI body weight were also gender-related (PIO-diet male PS1-KI versus PIO-diet female PS1-KI, P=0.028). Data are shown as means±S.E.M.; n=12 for each group. Statistical analysis was performed with analysis of variance for repeated measures. *PIO treatment effect; ^#genotype effect; ^§gender effect. P≤0.01 in all cases