Figure 5

PIO effects on complexes I and IV expression and activity in hippocampal mitochondria. Mitochondrial fractions from hippocampi (10 mg; wet weight) of treated and untreated animals were analyzed with BN-PAGE and subsequent histochemical in-gel staining to evaluate expression and activity of electron transport chain complexes I and IV. Panel a shows results in female mice, panel b shows results in males. Complex I results are in the upper part and complex IV in the lower part. For each complex, expression and activity data are shown along with representative blots. (a) PIO treatment induced significant reductions in expression of both complexes in WT animals. PIO induced a trend toward reduction in activity levels of complex I in PS1-KI mice (see also Supplementary Table S1). (b) PIO treatment induced significant decreased activity of complex I in WT animals. CTRL- and PIO-diet samples are shown with open and crossed bars, respectively. Band areas are expressed as absolute values (arbitrary units (A.U.)). Data are shown as means±S.E.M.; n=4 for each group. Statistical analysis was performed with two-tailed Student’s t-test for unpaired comparisons between the treated and untreated groups (95% confidence level). *0.05<P≤0.095; **0.05≤P<0.01; ***P≤0.01