Figure 2 | Cell Death & Disease

Figure 2

From: Stabilization of ribozyme-like cis-noncoding rRNAs induces apoptotic and nonapoptotic death in lung cells

Figure 2

Enrichment of sense nc-rRNAs and identification of 5′- and 3′-termini of sense nc-rRNAs. (a) The ratio of RT-PCR intensity between sense nc-rRNAs and the reference transcript, either the 18S rRNA or Gapdh, was used to calculate the fold enrichment (values inside the histogram) after magnetic purification treatment (T), compared with an aliquot of untreated crude RNA before the purification (UT). All enrichments were statistically significant. The linear scale in Y-axis represents transformed values using log (original ratio × 107). Error bars denote ±S.E. (b) Alignment of two amplified products (bottom sequences at left and right panels) to the BALB/c rDNA sequence (GenBank GU372691, top sequences, number in reference to transcription start site). The dotted/underlined CCG trinucleotides on the left panel cannot be assigned unambiguously to either upstream or downstream sequences. A differential single-nucleotide polymorphism is marked by the rectangular box in these two sequences. The T nucleotide at 277 on the right panel does not align with the rDNA sequence

Back to article page