Figure 4 | Cell Death & Disease

Figure 4

From: Stabilization of ribozyme-like cis-noncoding rRNAs induces apoptotic and nonapoptotic death in lung cells

Figure 4

The levels of nc-rRNAs, pre-rRNAs, and cell viability after LNA1-S and LNA1-AS treatments. (a) Abundant dead cells, represented by the dense granules under microscope, were immediately recognizable in cells 24 h after transfection with LNA1-S ( × 50 magnification). (b) The viable cells relative to vehicle treatment were also significantly reduced at 24 h after LNA treatments and the effect was more pronounced for LNA1-S, compared with LNA1-AS (*P<0.05; **P<0.0005). Error bars denote ±S.E. (c) Heat map for the nc-rRNA and pre-rRNA profiles in A5 and E9 cells at 48 h following two transfections of LNA gapmer with 24 h apart. Statistically significant changes in reference to corresponding transcripts in vehicle-treated adherent cells were colored in red for upregulation and in green for downregulation. Numbers indicate fold-changes and P-values. *P<0.05 for transcript differences between LNA1-S and LNA1-AS treatment in adherent and detached cells

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