Figure 6
The apoptotic responses of cells to additional LNAs and treatment schedule. (a) Effects at 24 h following single transfection of LNAs. The UL and UR fractions represent estimated apoptotic cell populations. The LL fraction contains nonapoptotic/fragmented cells and the LR region corresponds to unaffected cells (X-axis: propidium iodide intensity; Y-axis: fluorescein intensity in log scale). The steam-like appearance in the UL and UR fractions of LNA4-S-treated A5 cells indicates large apoptotic population. ‡P<0.05, #P<0.05, and *P<0.05, compared with LNA1-AS1, LNA2-AS, and LNA2-AS1, respectively. (b) The responses at 48 h following two transfections of LNAs with 24 h apart. *P<0.05 and **P<0.01, compared with vehicle control. (c) Heat map for the nc-rRNA and pre-rRNA profiles in A5 and E9 cells at 48 h following two transfections of LNA4-S gapmer. Statistically significant changes in reference to corresponding transcripts in vehicle-treated adherent cells were colored in red for upregulation and in green for downregulation. Numbers indicate fold-changes and P-values
