Figure 5 | Cell Death & Disease

Figure 5

From: Resistance to DNA-damaging treatment in non-small cell lung cancer tumor-initiating cells involves reduced DNA-PK/ATM activation and diminished cell cycle arrest

Figure 5

NSCLC TICS display reduced PARP cleavage and caspase 3 activation after IR and cisplatin treatment. (a and b) PARP cleavage was used as a marker of apoptosis in H125 TICs versus bulk cells. (a) Western blot analysis of PARP full-length and cleavage fragment, 1, 4 and 24 h after 8 Gy IR. (b) Western blot analysis of PARP full-length and cleavage fragment 24 h after 1 and 10 μM of continuous cisplatin treatment. A representative blot of two independent experiments is shown. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as a loading control. (c) Reduced activation of caspase 3 after 48 h of 1 and 10 μM continuous cisplatin in H125 and A549 TICs, as measured by flow cytometry. The threshold was arbitrarily adjusted to 5% for untreated cells. *P<0.05

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