Table 3 Sensitivity of UKF-NB-3 cells, UKF-NB-3rNutlin10 μM cells, UKF-NB-3rNutlin10 μM cells transduced with a lentiviral vector encoding wild-type p53 (UKF-NB-3rNutlin10 μMwtp53), and UKF-NB-3rNutlin10 μM cells transduced with a control vector (UKF-NB-3rNutlin10 μMcontrol) to RITA or nutlin-3 indicated by the concentration that reduces cell viability by 50% (IC50) indicated by MTT assay after a 5 day treatment period

From: Human neuroblastoma cells with acquired resistance to the p53 activator RITA retain functional p53 and sensitivity to other p53 activating agents

Cell line

RITA ( μ M)

Nutlin-3 ( μ M)

UKF-NB-3

0.11±0.02

1.47±0.15

UKF-NB-3rNutlin10 μM

0.62±0.04

32.83±2.91

UKF-NB-3rNutlin10 μMwtp53

0.24±0.03

5.02±1.73

UKF-NB-3rNutlin10 μMcontrol

0.58±0.03

36.19±3.08