Figure 4 | Cell Death & Disease

Figure 4

From: TIAF1 self-aggregation in peritumor capsule formation, spontaneous activation of SMAD-responsive promoter in p53-deficient environment, and cell death

Figure 4

TIAF1 self-aggregation is essential for causing spontaneous activation of a SMAD-responsive promoter in p53-deficient cells. (a) COS7 cells were transfected by liposome with plasmids for expressing ECFP, ECFP-Smad4, ECFP-TIAF1 and/or a GFP-reporting plasmid with a SMAD-responsive element.17, 35 In controls, cells were transfected with a negative or a positive plasmid. After 24 h, the cells were imaged by fluorescence microscopy. The extent of promoter activation was measured (mean±S.D.; Student’s t-test; n=3).17, 35 Smad4 induced the promoter activation, whereas TIAF1 suppressed the effect. Similar results were observed by tagging Smad4 or TIAF1 with DsRed (data not shown). neg, negative control; pos, positive control; null, electroporation with medium only. (b) p53-deficient NCI-H1299 cells were transfected with DsRed-TIAF1, TIAF1dm or ECFP-WOX1, in the presence of the SMAD promoter plasmid. DsRed-TIAF1 induced spontaneous activation of the SMAD-governed promoter, whereas the monomeric DsRed had no effect. A representative set of promoter activation is shown (right panel). Both negative and positive controls were included in the experiments (mean±S.D.; Student’s t-test; n=3)

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